HMGB1 Knockout cell line (LS 174T）

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HMGB1 Knockout cell line (LS 174T） Order

HMGB1 Knockout cell line (LS 174T）
Catalog#
YKO-H913
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HMGB1 Knockout cell line (LS 174T）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HMGB1 Knockout cell line (LS 174T）
Cell Morphology
Epithelial-like,adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
HMGB1
Gene ID
3146
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E0XI(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript HMGB1-203)
Validation: PCR+Sanger Sequencing
HMGB1 Knockout cell line (LS 174T） Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:LS 174T		 STR Info (Cell bank)
Cell Line:LS174T
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 1116111415.0
D13S317 10101010
D7S820 11111111
D16S539 11131113
VWA 151617.0151718.0
TH01 6767
AMEL XXXX
TPOX 8989
CSF1PO 10131014
D12S391 1620
FGA 202122.0
D2S1338 1822
D21S11 293031.0
D18S51 1113
D8S1179 1216
D3S1358 1517
D6S1043 121314.0
PENTAE 1516
D19S433 1415
PENTAD 1010
D1S1656 121318.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HMGB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LS 174T cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HMGB1 gene knockout HMGB1 Knockout cell line (LS 174T） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
HMGB1 Knockout cell line (LS 174T）
HMGB1 Knockout cell line (LS 174T）
Catalog#
YKO-H913
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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